Laboratory Safety Quiz
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1) What should you do when glass breaks in your lab?
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a. Evacuate the lab and call HazMat services.
b. Collect broken glass and place it into plastic sharps container.
c. Collect broken glass and dispose of it with regular trash.
d. Collect broken glass and place it into a cardboard container labeled “Broken glass”.
2) What should you do if a chemical gets in your eyes?
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a. Call 911 and wait for emergency personnel. Don’t do any treatment yourself.
b. Wash your eyes with plenty of water and seek medical attention.
c. Wash your eyes with 1% sodium bicarbonate.
d. Go to emergency room.
3) What should you do if you smell gas?
a. Turn off all electric equipment and call the gas company.
b. Locate the source of gas leak and correct the problem.
c. Call 911.
d. Evacuate the room and call the engineers. Do not turn electric devices on or off
4) What chemicals can be stored under the sink?
a. Regular chemicals, but not acids, bases, or flammables
b. Bleach and detergents
c. Chemicals insoluble in water
d. None of the above
5) What should you do with excess chemicals?
a. Offer them to colleagues in other labs through research administration.
b. Dispose of them with biohazardous waste.
c. Call HazMat to arrange removal.
d. A or C
6) Is eating and drinking allowed in the laboratory
a. Not allowed under any circumstances.
b. Snacks and drinks are allowed when working at your desk in the lab.
c. Drinking water is allowed.
d. Allowed if biohazards or radiochemicals are not used in the lab.
7) Which items are personal protective equipment (PPE)?
a. Medical scrubs
b. Sleeve protectors
c. Ear plugs
d. Shoes with rubber sole
8) Who is responsible for regular inspection of emergency eye wash stations?
a. Research Administration
b. Engineers
c. Contractor who installed the eyewash station
d. PI, lab safety officer, or his/her designee
9) At what intervals the eyewash station should be tested?
a. Daily
b. Weekly
c. Monthly
d. Once every 3 months
10) When should sharps containers be replaced?
a. Every 3 months or when they are full, whatever comes first.
b. When they are full.
c. When they are ¾ full.
d. At regular time intervals.
11) What is the proper use of germicidal UV lights?
a. Turn them on whenever equipment is not in use.
b. Turn them on when equipment is in use.
c. Turn them on for 15-20 min prior to experiment.
d. Turn them off 15-20 min before using the equipment to allow ozone dissipation.
e. C and D
12) UV light has the following effects:
a. Produces ozone
b. Ruins plastics
c. Causes skin burns (erythema), and may potentially lead to skin cancer
d. Causes damage to eyes
e. All of the above
13) Who must wear ear protection when using sources of ultrasound?
a. No need for ear protection because ultrasound frequency is outside human hearing range.
b. Only a person using the sonicator.
c. A person using the sonicator and people working nearby.
d. Only personnel using sonicators with power output over 20 W.
14) Which of the following is the lowest potentially lethal voltage?
a. 12V
b. 24V
c. 48V
d. 120V
15) What should be the type and rating of a replacement electric fuse?
a. Rating indicated on a device, or higher
b. Rating indicated on a device, or lower
c. Exact rating and type indicated on a device
d. Fuse type (fast or slow) does not matter as long as it has proper current rating
16) Which statement is false?
a. Liquid heated in an open glass vessel may suddenly burst.
b. Chemical glassware can be heated on open fire.
c. Boiling chips reduce the risk of liquid overheating.
d. Any lab glassware can be heated on hot plates.
17) Correct practices in using a fume hood include:
a. When working in a hood, keep the sash as wide open as possible to allow maximum air flow.
b. When the hood is not in use, leave the sash open.
c. A and B
d. None of the above
18) What chemicals below must be handled in a fume hood?
a. 37% formaldehyde solution
b. 100% beta-mercaptoethanol
c. 30% hydrogen peroxide
d. A and B
e. A, B, and C
19) Which of the following are good laboratory practices with regard to safety?
a. Keep chemicals in their original containers.
b. Record your experiments in a lab notebook.
c. Organize chemicals alphabetically.
d. All of the above
20) What should be done if a potentially hazardous chemical is spilled?
a. Put on appropriate PPE and clean up the spill as soon as possible.
b. Notify your PI and everybody in the room, and then clean up the spill.
c. Cover the spill with absorbent material if safe to do so, evacuate the room, close the doors, and notify safety officer.
d. None of the above
21) Latex gloves do not provide protection from:
a. Toxic chemicals
b. Acids
c. Sharps
d. Biohazardous agents
22) You need to weigh 5 mg of cycloheximide powder. The label on the bottle says "toxic". What PPE do you need for this task?
a. No PPE needed because the amount of cycloheximide to be handled is very small
b. Gloves
c. Gloves, dust mask, lab coat, and eye protection
d. Gloves, dust mask, lab coat, and apron
23) You have a centrifuge in your lab that works fine, however the lid interlock is erratic, sometimes allowing opening the lid when the rotor is turning. Can you use this centrifuge?
a. The centrifuge cannot be used before the problem is fixed
b. The centrifuge can be used
c. The centrifuge can be used, however, everybody in the lab must be aware of the problem
d. The centrifuge must be discarded
24) You need to move a gas cylinder 2 meters to the right from its current position. What is the correct way to do this job?
a. Because the distance is short, you can do it yourself. Unchain the cylinder, tilt it slightly, carefully roll it to the new position, and secure with chain to the wall.
b. Because the cylinder is heavy, ask someone in the lab to help you.
c. Use special dolly to do the job.
d. None of the above
25) You need to do an experiment with a metalloorganic compound that self-ignites upon contact with air. You don’t have much experience with metalloorganics, moreover, you don’t have much general lab experience. However, you have been shown several times how to handle metalloorganic compounds safely. You come to the lab on Saturday to do the experiment, but there is nobody in the lab or on the floor. What should you do?
a. Go ahead and do the experiment because you know well how to do it.
b. Call your supervisor, and if he or she does not object, do the experiment.
c. Do another experiment that does not involve metalloorganics.
d. Postpone the experiment until someone with expertise is present to help you
26) Emergency has been declared when you are in the middle of an experiment. Your action:
a. Finish your experiment ASAP and leave the building using a staircase.
b. Continue with your experiment. Most likely it is a false alarm.
c. Leave the building using a staircase. Stay away from the building and wait for directions.
d. Immediately leave the building using staircase or cargo elevator. Do not use passenger elevator because firefighters will need it.
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