User-Prepared Libraries
Please submit ≥ 20 µL of your undiluted 10 nM library suspended in 10 mM Tris-HCl, pH 8.5.
DNA Samples
Please submit ≥ 1 µg of high quality DNA with a concentration of > 50 ng/µL. DNA will be quantified using the Qubit 2.0 fluorometer.
RNA Samples
Please submit ≥ 1 µg of total RNA with a concentration of at least 50 ng/µL. RNA will be quantified using the Qubit 2.0 fluorometer and RNA integrity assessed using the Agilent 2100 Bioanalyzer.
All prepared libraries will be quality checked using the Agilent 2100 Bioanalyzer and the KAPA Library Quantification qPCR kit.